Open AccessEffect of heterologous expression of phaG [( R )‐3‐hydroxyacyl‐ACP‐CoA transferase] on polyhydroxyalkanoate accumulation from the aromatic hydrocarbon phenylacetic acid in Pseudomonas species
Author(s) -
Tobin Karen M.,
O'Leary Niall D.,
Dobson Alan D.W.,
O'Connor Kevin E.
Publication year - 2007
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00607.x
Subject(s) - phenylacetic acid , polyhydroxyalkanoates , pseudomonas , chemistry , pseudomonas putida , heterologous , biochemistry , transferase , hydrocarbon , heterologous expression , stereochemistry , microbiology and biotechnology , enzyme , organic chemistry , biology , recombinant dna , bacteria , genetics , gene
Five Pseudomonas strains capable of growth with the aromatic carboxylic acid phenylacetic acid were investigated with a view to improving PHA accumulation. The overexpression of ( R )‐3‐hydroxyacyl‐ACP‐CoA transferase (PhaG) from Pseudomonas putida CA‐3 increased PHA accumulation in only one of the five strains tested, namely Pseudomonas jessenii C8. Recombinant P. jessenii C8 harbouring the phaG gene showed a 4.1‐fold increase (9.6–39% cell dry weight) in PHA accumulation when grown on phenylacetic acid (15 mM) compared with the wild‐type strain. This is the highest reported level of PHA accumulation from phenylacetic acid. This is also the first time the heterologous expression of phaG has resulted in improved PHA accumulation from an aromatic carbon source. The growth patterns of the wild type and recombinant strains were very similar, with no significant differences observed in carbon and nitrogen utilization.