Open AccessRapid identification of Escherichia coli safety and laboratory strain lineages based on Multiplex‐PCR
Author(s) -
Bauer Andreas Peter,
Dieckmann Sarah Maria,
Ludwig Wolfgang,
Schleifer KarlHeinz
Publication year - 2007
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00594.x
Subject(s) - biology , multiplex polymerase chain reaction , escherichia coli , genome , polymerase chain reaction , salmonella , multiplex , computational biology , genetics , suppression subtractive hybridization , in silico , identification (biology) , microbiology and biotechnology , gene , bacteria , complementary dna , cdna library , botany
Escherichia coli K‐12, B, C and W strains are the most frequently used bacterial safety and laboratory strains. Lineage‐specific DNA fragments were detected by microplate subtractive hybridization and utilized to create a fast differentiation method using a single PCR reaction to differentiate clearly the four lineages and separate them from pathogenic variants. The method has been evaluated on a comprehensive selection of widely used laboratory strains and a variety of pathogenic E. coli representatives. In addition, in silico analysis on all available E. coli genomes and the genomes of the close relatives Shigella and Salmonella confirmed the reliability of the proposed method. A fast identification and differentiation of E. coli safety strains by Multiplex‐PCR is a useful tool for researchers and companies to check and monitor their reference stocks.