Open AccessIdentification of New World Leishmania species from Peru by biochemical techniques and multiplex PCR assay
Author(s) -
RodríguezGonzález Isabel,
Marín Clotilde,
Longoni Silvia S.,
Mateo Hector,
Alunda José M.,
Minaya Gloria,
GutiérrezSánchez Ramón,
Vargas Franklin,
SánchezMoreno Manuel
Publication year - 2007
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00574.x
Subject(s) - biology , cluster (spacecraft) , leishmania infantum , leishmania , multiplex polymerase chain reaction , leishmania chagasi , polymerase chain reaction , multiplex , genetics , visceral leishmaniasis , microbiology and biotechnology , virology , gene , leishmaniasis , parasite hosting , computer science , world wide web , programming language
We have characterized diverse strains or species of Leishmania isolated in humans that are currently circulating throughout Peru, by means of isoenzymatic characterization, kDNA analysis by restriction enzymes, and multiplex PCR assay. The cluster analysis gave five groups. Cluster 1 includes L . ( L. ) donovani together with the isolates LP4 and LP7, forming the donovani complex. Thus, this complex corresponds to the New World visceral form, L . ( L. ) chagasi . Cluster 2 is formed by the isolates LP1–LP3, LP6, LP10, LP9, and LP11, phylogenetically intermediate between Cluster 1 and Cluster 3, or they can be treated as hybrids. Cluster 3 is divided into two subgroups: one formed by L . ( V. ) peruviana , together with the isolates LP14 and LP5, and the second one formed by L . ( V .) brazilensis and the isolate LP8. These two subgroups form part of the brazilensis complex. The three strains of L . ( L .) infantum [ L . ( L .) infantum I and II and la LSI] make up Cluster 4. In Cluster 5, we include the three Mexican strains (LM1–LM3) forming one subgroup while we would place L . ( L .) amazonensis in another subgroup. These two subgroups would comprise the complex mexicana .