Open AccessIsolation and characterization of a new bacterium capable of biotransforming cis ‐epoxysuccinic acid to d (−)‐tartaric acid
Author(s) -
Li Xia,
Ma Xiaohang,
Zhao Yuhua,
Jia Xiaoming,
Kai Lei,
Guo Kangping,
Zhao Weifeng
Publication year - 2007
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00552.x
Subject(s) - isolation (microbiology) , bacteria , tartaric acid , chemistry , biochemistry , microbiology and biotechnology , biology , genetics , citric acid
A bacterial strain 1–3 capable of enantioselectively hydrolyzing cis ‐epoxysuccinic acid to d (−)‐tartaric acid was isolated from soil. Phenotypic characteristics, Biolog GN test and phylogenetic analysis of strain 1–3 indicated that it belongs to the genus Bordetella . cis ‐Epoxysuccinic acid could be used as a carbon source and inducer for production of cis ‐epoxysuccinic acid hydrolase. After the purification of the enzymatic biotransformed product, a colorless and scentless crystal was obtained, which was confirmed to be the tartaric acid with laevorotatory optical rotation by the 1 H nuclear magnetic resonance spectrum and optical rotation analysis.