Open AccessOrganization of a partial S10 operon and its transcriptional analysis in Flavobacterium hibernum strain W22
Author(s) -
Chen Shicheng,
Bagdasarian Michael,
Kaufman Michael G.,
Walker Edward D.
Publication year - 2007
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00543.x
Subject(s) - operon , strain (injury) , flavobacterium , microbiology and biotechnology , biology , genetics , bacteria , gene , anatomy , pseudomonas , escherichia coli
A cluster of six genes from Flavobacterium hibernum strain W22, fus‐rpsJ‐rplC‐rplD‐rplW‐rplB , was cloned and sequenced. A short fragment upstream of rplC , but not of rpsJ , showed strong promoter activity in flavobacteria. TATCTTTG and TTG motifs that are conserved in Flavobacterium promoters were found immediately upstream of the transcription start point of rplC , at the −7 and −33 positions, respectively. RT‐PCR analysis of the transcripts revealed that rpsJ and rplC are expressed as separate transcriptional units, whereas rplC and rplD‐rplW‐rplB are cotranscribed as a single mRNA, in contrast to the situation in the Gammaproteobacteria , which have a single transcriptional unit of 11 ribosomal S10 genes.