Open AccessCloning of mpd gene from a chlorpyrifos‐degrading bacterium and use of this strain in bioremediation of contaminated soil
Author(s) -
Yang Chao,
Liu Na,
Guo Xinmin,
Qiao Chuanling
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00478.x
Subject(s) - bioremediation , stenotrophomonas , bacteria , 16s ribosomal rna , chlorpyrifos , parathion methyl , strain (injury) , parathion , microbiology and biotechnology , chemistry , biology , food science , pesticide , genetics , agronomy , anatomy
An effective chlorpyrifos‐degrading bacterium (named strain YC‐1) was isolated from the sludge of the wastewater treating system of an organophosphorus pesticides manufacturer. Based on the results of phenotypic features, phylogenetic similarity of 16S rRNA gene sequences and BIOLOG test, strain YC‐1 was identified as the genus Stenotrophomonas . The isolate utilized chlorpyrifos as the sole source of carbon and phosphorus for its growth and hydrolyzed chlorpyrifos to 3,5,6‐trichloro‐2‐pyridinol. Parathion, methyl parathion, and fenitrothion also could be degraded by strain YC‐1 when provided as the sole source of carbon and phosphorus. The gene encoding the organophosphorus hydrolase was cloned using a PCR cloning strategy based on the known methyl parathion degrading ( mpd ) gene of Plesiomonas sp. M6. Sequence blast result indicated this gene has 99% similar to mpd . The inoculation of strain YC‐1 (10 6 cells g −1 ) to soil treated with 100 mg kg −1 chlorpyrifos resulted in a higher degradation rate than in noninoculated soils. Theses results highlight the potential of this bacterium to be used in the cleanup of contaminated pesticide waste in the environment.