Open AccessChaperone protection of immature molybdoenzyme during molybdenum cofactor limitation
Author(s) -
Genest Olivier,
Seduk Farida,
Théraulaz Laurence,
Méjean Vincent,
IobbiNivol Chantal
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00468.x
Subject(s) - molybdenum cofactor , cofactor , molybdenum , proteolysis , chaperone (clinical) , escherichia coli , limiting , biology , chemistry , biochemistry , medicine , enzyme , gene , pathology , inorganic chemistry , mechanical engineering , engineering
Maturation of molybdoenzyme TorA involves chaperone TorD. This study shows that TorD is also required to protect apoTorA against proteolysis when the molybdenum cofactor is limiting in Escherichia coli . The absence of TorD leads to a complete loss of apoTorA during molybdenum cofactor deficiency whereas the presence of TorD maintains a significant amount of apoTorA that can be matured when the molybdenum cofactor becomes available.