Open AccessEffect of sulfur‐containing compounds on Bacillus cellulosome‐associated ‘CMCase’ and ‘Avicelase’ activities
Author(s) -
Beukes Natasha,
Pletschke Brett Ivan
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00465.x
Subject(s) - cellulosome , cellulase , chemistry , biochemistry , clostridium thermocellum , bacillus (shape) , cellulose , bacillus pumilus , cellulosic ethanol , microbiology and biotechnology , bacteria , biology , genetics
The isolation of cellulosomes from clostridial sources has been extensively studied; however, the isolation of cellulosomes from facultative soil anaerobes of the family Bacillaceae is not as well characterized. The Bacillus cellulosome (celluloxylanosome) essentially consists of two complex components: C‐I and C‐II. This multi‐component complex enables Bacillus to degrade a variety of carbonaceous compounds as it is composed of several enzymes, such as cellulases, xylanases and other degradative enzymes. The cellulosomal cellulases from Bacillus megaterium were purified using cellulose affinity chromatography, followed by Sepharose 4B gel filtration chromatography. The objective of this investigation was to establish the effect of sulfate and sulfide on cellulosomal ‘cellulase’ activity. An increase in sulfide concentration led to a general enhancement of cellulosomal‐associated cellulolytic activity, whereas an increase in sulfate concentration resulted in an inhibition of the cellulosome‐associated cellulolytic activity.