Open AccessNature of DNA‐bound fatty acids in Pseudomonas aurantiaca
Author(s) -
Zhdanov Renat I.,
Shmyrina Anastasia S.,
Zarubina Tatyana V.,
Mulyukin Andrey L.,
ElRegistan Galina I.,
Haupt Nicole,
Kraus Angelika,
Lorenz Wilhelm
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00461.x
Subject(s) - biochemistry , dna , fatty acid , free fatty acid receptor , biology , chemistry , polyunsaturated fatty acid
The existence of Pseudomonas aurantiaca DNA‐bound fatty acids and lipids is presented in this work. The isolation of DNA was carried out by two different procedures, namely, phenol and detergent‐based phenol isolation in order to prove the presence of DNA‐bound lipids. The lipid content of DNA is expressed in terms of fatty acid profile. A high level of 16:0, 18:0 and 18:1 is characteristic for tightly bound DNA lipids. On the other hand, the fatty acids such as 14:1, iso14:0 and iso16:0 are found in trace amounts only in DNA lipid fraction, but these fatty acids are not found in the whole‐cell lipids. Absolutely no 3‐hydroxy fatty acids were found in DNA lipids. However, both C16 and C18 species represent the main fatty acids of whole‐cell and DNA‐bound lipids. The presence of DNA‐bound lipids even under tough treatment of DNA allows to conclude that these lipids represent a special pool among cellular lipids.