Open AccessDevelopment of an ex vivo organ culture model using human gastro‐intestinal tissue and Campylobacter jejuni
Author(s) -
Grant Andrew J.,
Woodward Jeremy,
Maskell Duncan J.
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00449.x
Subject(s) - ex vivo , campylobacter jejuni , organ culture , microbiology and biotechnology , biology , in vivo , pathogen , multicellular organism , bacteria , cell , in vitro , genetics
Campylobacter jejuni is an important food‐borne pathogen. However, relatively little is understood regarding its pathogenesis, and research is hampered by the lack of a suitable model. Recently, a number of groups have developed assays to study the pathogenic mechanisms of C. jejuni using cell culture models. Here, we report the development of an ex vivo organ culture model, allowing for the maintenance of intestinal mucosal tissue, to permit more complex host–bacterium interactions to be studied. Ex vivo organ culture highlights the propensity for C. jejuni to adhere to mucosal tissue via the flagellum, either as discrete colonies or as multicellular units.