Open AccessMolecular origin of two polysaccharides of Campylobacter jejuni 81116
Author(s) -
Kilcoyne Michelle,
Moran Anthony P.,
Shashkov Alexander S.,
Senchenkova Sof'ya N.,
Ferris John A.,
Corcoran Adrian T.,
Savage Angela V.
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00436.x
Subject(s) - polysaccharide , campylobacter jejuni , microbiology and biotechnology , chemistry , gel permeation chromatography , campylobacter , lipopolysaccharide , bacteria , centrifugation , biochemistry , biology , organic chemistry , genetics , endocrinology , polymer
The nature of the polysaccharide molecules of the human enteric pathogen Campylobacter jejuni has been the subject of debate. Previously, C. jejuni 81116 was shown to contain two different polysaccharides, one acidic (polysaccharide A) and the other neutral (polysaccharide B), occurring in a 3 : 1 ratio, respectively. The aim of this study was to determine the molecular origin of these polysaccharides. Using a combination of centrifugation, gel permeation chromatography, chemical assays, and 1 H‐NMR analysis, polysaccharide B was shown to be derived from lipopolysaccharide and polysaccharide A from capsular polysaccharide. Thus, C. jejuni 81116 produces both lipopolysaccharide‐like molecules and capsular polysaccharide.