Open AccessMolecular serotype identification of Streptococcus agalactiae of bovine origin by multiplex PCR‐based reverse line blot (mPCR/RLB) hybridization assay
Author(s) -
Zhao Zuotao,
Kong Fanrong,
Martinez Gabriela,
Zeng Xianyu,
Gottschalk Marcelo,
Gilbert Gwendolyn L.
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00428.x
Subject(s) - streptococcus agalactiae , serotype , biology , multiplex polymerase chain reaction , microbiology and biotechnology , multiplex , streptococcus , polymerase chain reaction , virology , gene , genetics , bacteria
We used a multiplex PCR‐based reverse line blot (mPCR/RLB) hybridization assay and sequencing of a variable region of the cps cluster to identify serotypes of 140 Streptococcus agalactiae (group B Streptococcus ; GBS) isolates from cattle. Only 71 (51%) isolates were typeable using antisera, but molecular serotypes (MS) were assigned to 133 (95%) and 139 (99%) isolates by partial cpsE‐cpsF‐cpsG sequencing and mPCR/RLB, respectively. Ninety‐four isolates (67%) belonged to MS III and most belonged to a molecular serosubtype (msst) III‐3, which is uncommon among GBS isolates from humans. Our results demonstrate that cps clusters of bovine GBS differ significantly from those of GBS isolates from humans.