Open AccessRole of two 2‐oxoglutarate:ferredoxin oxidoreductases in Hydrogenobacter thermophilus under aerobic and anaerobic conditions
Author(s) -
Yamamoto Masahiro,
Arai Hiroyuki,
Ishii Masaharu,
Igarashi Yasuo
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00415.x
Subject(s) - thermophile , ferredoxin , mutant , biochemistry , anaerobic exercise , biology , alpha ketoglutarate , bacteria , microbiology and biotechnology , enzyme , chemistry , gene , genetics , physiology
Hydrogenobacter thermophilus TK‐6 is a thermophilic, hydrogen‐oxidizing bacterium that fixes carbon dioxide as a sole carbon source via the reductive tricarboxylic acid cycle. 2‐Oxoglutarate:ferredoxin oxidoreductase (OGOR) is one of the key enzymes in the pathway. Strain TK‐6 has at least two isozymes of OGOR, namely For and Kor. These OGORs showed different expression patterns under aerobic conditions than under anaerobic conditions. In this work, we developed a homologous recombination method for Hydrogenobacter , and constructed a For mutant and a Kor mutant. Observation of phenotypes of the mutant strains showed that Kor was essential for anaerobic growth and that For activity supported robust aerobic growth of the organism.