Open AccessInfluence of environmental conditions on putisolvins I and II production in Pseudomonas putida strain PCL1445
Author(s) -
Dubern JeanFrédéric,
Bloemberg Guido V.
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00406.x
Subject(s) - pseudomonas putida , swarming motility , strain (injury) , biosynthesis , chemistry , mutant , biochemistry , food science , pseudomonas , biofilm , pseudomonadales , salt (chemistry) , growth medium , biology , bacteria , enzyme , quorum sensing , organic chemistry , gene , genetics , anatomy , virulence
Pseudomonas putida strain PCL1445 produces the cyclic lipopeptides putisolvin I and II, which are biosurfactants affecting environmentally important functions including swarming motility, solubility of nutrients and biofilm formation and maintenance. In this study, the effect of relevant nutritional and environmental factors on putisolvin production by PCL1445 was analyzed. Growth and levels of putisolvins I and II biosynthesis were significantly influenced by various carbon sources and by the nature of amino acid supplemented to the culture medium. An increase in the salt concentration of BM medium as well as growth at 1% oxygen significantly increased putisolvin production. In addition, we recently demonstrated that growth at a low temperature has a positive effect on putisolvin production. Analysis of the expression of gacA and gacS in Tn5 luxAB mutant strains at a low temperature or under a high salt concentration suggests that biosynthesis of putisolvin is modulated through the GacA/GacS two‐component regulatory system in PCL1445 under the tested conditions.