Open AccessMicroarray expression profiling of Yersinia pestis in response to chloramphenicol
Author(s) -
Qiu Jingfu,
Zhou Dongsheng,
Qin Long,
Han Yanping,
Wang Xiaoyi,
DU Zongmin,
Song Yajun,
Yang Ruifu
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00394.x
Subject(s) - yersinia pestis , gene , biology , chloramphenicol , gene expression profiling , genetics , microarray analysis techniques , microarray , gene expression , virulence , bacteria
Plague is a deadly disease caused by Yersinia pestis . Human plague can be effectively controlled by timely antibiotic administration, chloramphenicol being a drug of choice. In this study, a DNA microarray was used to investigate the gene expression profile of Y. pestis in response to chloramphenicol. Seven hundred and fifty‐five genes were differentially expressed on chloramphenicol treatment: 364 genes were up‐regulated and 391 were down‐regulated. In addition to a large number of genes encoding unknown or unassigned functions, genes encoding the components of the translation apparatus, cell envelope and transport/binding functions were strongly represented amongst the induced genes. Genes encoding proteins involved in energy metabolism and synthesis and modification of macromolecules were strongly represented amongst the down‐regulated genes. A number of heat‐shock genes were also repressed. These global transcriptional changes provide an insight into the mechanisms of action of chloramphenicol against Y. pestis .