Open AccessImmune response and protection by DNA vaccines expressing antigen 85B of Mycobacterium tuberculosis
Author(s) -
Pardini Manuela,
Giani Federico,
Palma Carla,
Iona Elisabetta,
Cafaro Aurelio,
Brunori Lara,
Rinaldi Monica,
Fazio Vito Michele,
Laguardia Maria Elena,
Carbonella Damiano Cosimo,
Magnani Mauro,
Ensoli Barbara,
Fattorini Lanfranco,
Cassone Antonio
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00390.x
Subject(s) - plasmid , dna vaccination , mycobacterium tuberculosis , immune system , biology , antigen , dna , virology , tuberculosis , microbiology and biotechnology , tuberculosis vaccines , immunology , medicine , genetics , pathology
A plasmid DNA containing two different expression cassettes was prepared to independently drive antigen 85B (85B) of Mycobacterium tuberculosis and HIV‐Tat in C57BL/6 mice. In vivo expression of the plasmid was demonstrated by efficient transcription of 85B and Tat mRNAs in mouse fibroblasts. DNA‐85B or DNA‐(85B‐Tat) were immunogenic and protected mice to the same extent against M. tuberculosis infection, with a decrease in the numbers of CFU lung −1 in comparison with nonimmunized animals down to levels (0.64 log10 CFU) not significantly different from protection conferred by bacillus Calmette‐Guérin vaccine (0.97 log10 CFU decrease). Multipromoter plasmids, which permit the reduction of the total amount of DNA injected, can be useful for DNA vaccination against tuberculosis.