Open AccessComparative proteomic analysis between the invasive and commensal strains of Staphylococcus epidermidis
Author(s) -
Yang XiaoMei,
Li Na,
Chen JieMin,
Ou YuanZhu,
Jin Hong,
Lu HaoJie,
Zhu YuLi,
Qin ZhiQiang,
Qu Di,
Yang PengYuan
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00327.x
Subject(s) - staphylococcus epidermidis , microbiology and biotechnology , biology , staphylococcus , commensalism , micrococcaceae , staphylococcus aureus , bacteria , antibacterial agent , antibiotics , genetics
Staphylococcus epidermidis is one of the major causative agents for nosocomial infections. To reveal the pathogenesis factors, we performed the comparative proteomic analysis of invasive ATCC35984 and commensal ATCC12228 strains by two‐dimensional gel electrophoresis and matrix‐assisted laser desorption/ionization‐time of flight‐mass spectrometry. The differentially expressed proteins were involved in carbohydrate metabolism, sugar binding, lipid degradation and amino acid binding. In addition, we demonstrated that the trap gene was transcribed by 3.657±0.156 ( P <0.01) ‐fold higher in ATCC35984 than in ATCC12228. Levels of accumulation‐associated protein (AAP) were found to be low by the immuno‐dot blotting assay in ATCC12228, which is unable to form biofilm. Our results suggest that the target of RNAIII activating protein and AAP may contribute to S. epidermidis virulence and biofilm formation.