Open AccessIsolation and characterization of functional insertion sequences of rhizobia
Author(s) -
HernandezLucas Ismael,
RamirezTrujillo Jose Augusto,
Gaitan Miguel Angel,
Guo Xianwu,
Flores Margarita,
MartinezRomero Esperanza,
PerezRueda Ernesto,
Mavingui Patrick
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00319.x
Subject(s) - rhizobia , biology , sinorhizobium meliloti , rhizobium , genome , genetics , insertion sequence , in silico , rhizobiaceae , bacteria , transposable element , computational biology , gene , symbiosis
Rhizobia are a group of bacteria that form nodules on the roots of legume host plants. The sequenced genomes of the rhizobia are characterized by the presence of many putative insertion sequences (IS) elements. However, it is unknown whether these IS elements are functional and it is therefore relevant to assess their transposition activity. In this work, several functional insertion sequences belonging to the IS 1256 , IS 3 , IS 5 , IS 166 , and IS 21 families were captured from Rhizobium tropici , Rhizobium sp. NGR234 and Sinorhizobium meliloti , using pGBG1 as a trapping system. In silico analysis shows that homologs of rhizobia mobile elements are present in distantly related genomes, suggesting that Rhizobium IS elements are prone to genetic transfer.