Open AccessDevelopment and application of a novel and effective screening method for aerobic denitrifying bacteria
Author(s) -
Kong QinXin,
Wang XinWei,
Jin Min,
Shen ZhiQiang,
Li JunWen
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00306.x
Subject(s) - denitrifying bacteria , aerobic denitrification , nitrite , bacteria , 16s ribosomal rna , microbiology and biotechnology , pseudomonas , biology , nitrate , nitrite reductase , food science , denitrification , chemistry , biochemistry , nitrogen , genetics , ecology , organic chemistry
Herein we describe a novel and effective screening method for aerobic denitrifying bacteria. For this procedure, we utilized KCN to inhibit the electron transference from Cytaa3 to oxygen in the bacteria respiratory chain. We employed a 3‐h aeration operation cycle and intermittent rotations. The resultant bacterial suspensions were plated on a KCN‐screening medium and incubated aerobically. Single colonies were selected and incubated in an aerobic culture medium. Culture nitrate and nitrite levels were determined over time, and ultimately four bacterial strains that performed denitrifying under aerobic conditions were identified by this method. Of these, strain Y2‐1‐1 demonstrated the best aerobic denitrifying ability. In a 5‐day test, the NO 3 − ‐N of the aerobic culture medium was reduced from 282.0±8.3 mg L −1 to 149.2±17.1 mg L −1 , with little nitrite or N 2 O production. The morphological, physiological and biochemical characteristics and the 16S rRNA gene sequence homology comparison data for this strain were consistent with the classification of the genus Pseudomonas . We named this strain Pseudomonas sp. Y2‐1‐1.