Open AccessThe effects of α‐lactalbumin and glycomacropeptide on the association of CaCo‐2 cells by enteropathogenic Escherichia coli, Salmonella typhimurium and Shigella flexneri
Author(s) -
Brück Wolfram M.,
Kelleher Shan L.,
Gibson Glenn R.,
Graverholt Gitte,
Lönnerdal Bo L.
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00268.x
Subject(s) - enteropathogenic escherichia coli , shigella flexneri , microbiology and biotechnology , shigella , salmonella , escherichia coli , alpha lactalbumin , enterobacteriaceae , biology , chemistry , bacteria , food science , lactalbumin , biochemistry , genetics , gene
Two milk components, α‐lactalbumin (α‐La) and glycomacropeptide (GMP) may inhibit intestinal infection/intoxification. 3 [H] thymidine‐labeled enteropathogenic Escherichia coli (EPEC), Salmonella typhimurium (ATCC 6994) or Shigella flexneri (ATCC 9199) were introduced to CaCo‐2 cultures and their association with CaCo‐2 cells was assessed. Undigested, pepsin‐digested and pepsin‐ and pancreatin‐digested α‐lactalbumin and glycomacropeptide inhibited association. Thus, milk supplemented with α‐lactalbumin and glycomacropeptide might be effective in inhibiting associations of the pathogens EPEC, Salmonella typhimurium , and Shigella flexneri to intestinal cells.