Open AccessIdentification of novel HrpXo regulons preceded by two cis ‐acting elements, a plant‐inducible promoter box and a −10 box‐like sequence, from the genome database of Xanthomonas oryzae pv. oryzae
Author(s) -
Furutani Ayako,
Nakayama Takeshi,
Ochiai Hirokazu,
Kaku Hisatoshi,
Kubo Yasuyuki,
Tsuge Seiji
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00265.x
Subject(s) - xanthomonas oryzae , xanthomonas oryzae pv. oryzae , orfs , biology , regulon , xanthomonas , gene , genetics , genome , promoter , open reading frame , peptide sequence , regulation of gene expression , gene expression
A regulatory protein HrpXo of Xanthomonas oryzae pv. oryzae , the causal agent of bacterial leaf blight of rice, is known to control the expression of hrp genes that encode components of a type III secretion system and of some effector protein genes. In this study, we screened novel HrpXo regulons from the genome database of X. oryzae pv. oryzae , searching for ORFs preceded by two predicted sequence motifs, a plant‐inducible promoter box‐like sequence and a −10 box‐like sequence. Using a gus reporter system, nine of 15 ORF candidates were expressed HrpXo dependently. We also showed by base‐substituted mutagenesis that both motifs are essential for the expression of the genes.