Open AccessS ‐adenosylmethionine activates adpA transcription and promotes streptomycin biosynthesis in Streptomyces griseus
Author(s) -
Shin SuKyoung,
Xu Delin,
Kwon HyungJin,
Suh JooWon
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00246.x
Subject(s) - streptomyces griseus , transcription (linguistics) , biology , mutant , reversion , psychological repression , streptomyces coelicolor , streptomycetaceae , heterologous , streptomycin , transcription factor , heterologous expression , streptomyces , gene , genetics , gene expression , actinomycetales , bacteria , phenotype , antibiotics , recombinant dna , linguistics , philosophy
S ‐adenosylmethionine (SAM), the major methyl donor in diverse biological processes, was previously documented as a regulator for secondary metabolism in Streptomyces . The present study demonstrates that exogenous SAM, in a quantity as low as 10 μM, enhanced streptomycin production and adpA transcription in both Streptomyces griseus wild‐type strain and mutant HO1, which displays no ArpA repression on the adpA promoter. SAM also enhanced xylE expression driven by the promoter of adpA or strR in a heterologous host, S. lividans . This report substantiates that exogenous SAM promotes adpA transcription in S. griseus , which is involved in the SAM‐mediated promotion of streptomycin, and that the mechanism underlying this event is shared in S. lividans .