Open AccessComparison of Vibrio harveyi strains isolated from shrimp farms and from culture collection in terms of toxicity and antibiotic resistance
Author(s) -
Nakayama Tatsuya,
Ito Emi,
Nomura Nakao,
Nomura Nobuhiko,
Matsumura Masatoshi
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00225.x
Subject(s) - vibrio harveyi , microbiology and biotechnology , kanamycin , biology , oxytetracycline , antibiotics , minimum inhibitory concentration , carbenicillin , antibiotic resistance , toxicity , subculture (biology) , vibrio , minimum bactericidal concentration , ampicillin , shrimp , bacteria , chemistry , fishery , genetics , organic chemistry
Vibrio harveyi strains isolated from shrimp farms (wild strains) were compared with those from culture collections in terms of minimum inhibitory concentration (MIC) and toxicity. Wild strains had higher MIC values for four antibiotics (kanamycin, carbenicillin, oxytetracycline and ampicillin) and also showed higher toxicity compared with culture collection strains. Vibrio harveyi with the lowest antibacterial resistance was chosen to test if a gradual increase in antibiotic concentration and frequent subculture would enhance its antibiotic resistance. Results showed that V. harveyi was able to develop resistance to oxytetracycline. The MIC value was 250 times higher compared with the MIC before subculturing. Moreover, the V. harveyi strain developed slightly higher toxicity. Therefore, it is possible that there is a relationship between antibiotic resistance and toxicity in V. harveyi .