Open AccessLeucocins 4010 from Leuconostoc carnosum cause a matrix related decrease in intracellular pH of Listeria monocytogenes
Author(s) -
Fang Weihuan,
Budde Birgitte Bjørn,
Siegumfeldt Henrik
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00219.x
Subject(s) - listeria monocytogenes , bacteriocin , nisin , agar , microbiology and biotechnology , chemistry , intracellular , bacteria , agar plate , food science , biology , biochemistry , antimicrobial , genetics
A mixed culture of single cells of Listeria monocytogenes and the bacteriocin producing Leuconostoc carnosum 4010 showed growth inhibition of L. monocytogenes , although the intracellular pH (pH i ) of L. monocytogenes followed by fluorescence ratio imaging microscopy was not affected. Furthermore, L. monocytogenes was exposed to the bacteriocins leucocins 4010 and nisin either in a liquid filled chamber or on the surface of an agar containing bacteriocins. Both bacteriocins caused dissipation of the pH gradient in L. monocytogenes and the effect was clearly dependent on the matrix, as the decrease in pH i occurred much more rapidly in liquid than in agar.