Open AccessThe WD‐40 repeat protein PkwA of Thermomonospora curvata is associated with rapid growth and is localized in the tips of growing hyphae
Author(s) -
Petříčková Kateřina,
Hašek Jiří,
Benada Oldřich,
Petříček Miroslav
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00215.x
Subject(s) - hypha , thermophile , biochemistry , biology , intracellular , protein kinase a , membrane protein , bacteria , chemistry , microbiology and biotechnology , botany , kinase , membrane , enzyme , genetics
The PkwA protein of the thermophilic actinomycete Thermomonospora curvata has already been reported as the first instance of a WD‐40 module‐containing protein of prokaryotic origin. This protein is composed of an N‐terminal eukaryotic‐type protein kinase domain and of seven C‐terminal WD‐40 repeats. PkwA is a peripheral membrane protein that is linked to the early exponential growth phase of the bacterium. Its intracellular concentrations are extremely low. We have shown that the protein forms high molecular weight complexes and is localized mainly in the tips of the young Thermomonospora vegetative hyphae.