Open AccessAeromonas sobria hemolysin causes diarrhea by increasing secretion of HCO 3 −
Author(s) -
Takahashi Akira,
Nakano Masayuki,
Okamoto Keinosuke,
Fujii Yoshio,
Mawatari Kazuaki,
Harada Nagakatsu,
Nakaya Yutaka
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00204.x
Subject(s) - microbiology and biotechnology , hemolysin , secretion , diarrhea , aeromonas , traveler's diarrhea , vibrionaceae , chemistry , biology , bacteria , medicine , virulence , biochemistry , genetics , gene
Aeromonas sobria hemolysin (ASH) is one of the major virulence factors produced by A. sobria , a causative agent of diarrhea in humans. We investigated the effects of ASH on anion transport in human colonic epithelial cells. ASH increased short circuit currents across the intestinal epithelia, which were suppressed by anion channel antagonists, such as carbonic anhydrase inhibitors, and by the removal of external HCO 3 − . Iliac fluid accumulation was also inhibited by carbonic anhydrase inhibitors. The results suggest that ASH activates HCO 3 − secretion, whose level correlates with the severity of diarrhea.