Open AccessRecognition of pore‐forming colicin Y by its cognate immunity protein
Author(s) -
Šmajs David,
Matějková Petra,
Weinstock George M.
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00201.x
Subject(s) - colicin , immunity , gene , biology , transmembrane domain , peptide sequence , mutagenesis , genetics , amino acid , escherichia coli , immune system , mutation
Construction of hybrid immunity genes between colicin U ( cui ) and Y ( cyi ) immunity genes and site‐directed mutagenesis of cyi were used to identify amino‐acid residues of the colicin Y immunity protein (Cyi) involved in recognition of colicin Y. These amino‐acid residues were localized close to the cytoplasmic site of the Cyi transmembrane helices T3 (S104, S107, F110, A112) and T4 (A159). Mutations in cui , which converted Cui sequence to Cyi sequence in positions 104, 107, 110, 112 and 159, resulted in an immunity gene that also conferred (besides immunity to colicin U) a high degree of immunity to colicin Y.