Open AccessSecretion of psychrophilic α‐amylase deletion mutants in Pseudoalteromonas haloplanktis TAC125
Author(s) -
Cusano Angela Maria,
Parrilli Ermenegilda,
Duilio Angela,
Sannia Giovanni,
Marino Gennaro,
Tutino Maria Luisa
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00193.x
Subject(s) - psychrophile , pseudoalteromonas , secretion , mutant , biology , microbiology and biotechnology , genetics , biochemistry , bacteria , gene , 16s ribosomal rna
The nature and location of structural features responsible for the secretion of a cold‐adapted α‐amylase in the Antarctic marine bacterium Pseudoalteromonas haloplanktis TAC125 was studied by deletion mutagenesis of the wild‐type enzyme and of chimerical proteins derived from the fusion of the α‐amylase with a reporter enzyme. Domain C of the psychrophilic α‐amylase contains secretion features involved in extracellular targeting.