Open AccessMolecular characterization of β‐lactamase genes blaA and blaB of Yersinia enterocolitica biovar 1A
Author(s) -
Sharma Sachin,
Mittal Shilpi,
Mallik Sarita,
Virdi Jugsharan S.
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00191.x
Subject(s) - biovar , yersinia enterocolitica , biology , microbiology and biotechnology , genetics , polymerase chain reaction , gene , homology (biology) , enterobacteriaceae , restriction enzyme , bacteria , escherichia coli
The β‐lactamase genes blaA and blaB were detected by PCR amplification in strains of Yersinia enterocolitica biovar 1A isolated from India, Germany, France and the USA. Both genes were detected in all strains. Polymerase chain reaction‐restriction fragment length polymorphism revealed genetic heterogeneity in blaA but not in blaB . Cluster analysis of blaA restriction profiles grouped the strains into three groups. The blaA gene of Y. enterocolitica biovar 1A showed a high degree of sequence homology to that of Y. enterocolitica 8081 (biovar 1B) and Y. enterocolitica Y‐56 (biovar 4), whereas homology was low with class A β‐lactamase genes of other members of the family Enterobacteriaceae . The p I 8.7 of enzyme Bla‐A of Y. enterocolitica biovar 1A was similar to that of biovars 2, 3 and 4. The enzyme Bla‐B focused at 6.8 and 7.1, indicating that biovar 1A strains produced a ‘B‐like’ enzyme. This is the first study to have investigated the genetic heterogeneity of the β‐lactamase genes of Y. enterocolitica .