Open AccessMolecular analysis of the bacterial community in a continental high‐temperature and water‐flooded petroleum reservoir
Author(s) -
Li Hui,
Yang ShiZhong,
Mu BoZhong,
Rong ZhaoFeng,
Zhang Jie
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00149.x
Subject(s) - gammaproteobacteria , betaproteobacteria , biology , 16s ribosomal rna , bacteria , proteobacteria , library , microbial population biology , mesophile , microbiology and biotechnology , ecology , actinobacteria , genetics
Water from a continental high‐temperature, long‐term water‐flooded petroleum reservoir in Huabei Oilfield in China was analysed for its bacterial community and diversity. The bacteria were characterized by their 16S rRNA genes. A 16S rRNA gene clone library was constructed from the community DNA, and using restriction fragment length polymorphism analysis, 337 randomly selected clones were clustered with 74 operational taxonomic units. Sequencing and phylogenetic analyses showed that the screened clones were affiliated with Gammaproteobacteria (85.7%), Thermotogales (6.8%), Epsilon proteobacteria (2.4%), low‐G+C Gram‐positive (2.1%), high‐G+C Gram‐positive, Beta proteobacteria and Nitrospira (each <1.0%). Thermopilic bacteria were found in the high‐temperature water from the flooded petroleum reservoir, as well as mesophilic bacteria such as Pseudomonas ‐like clones. The mesophilic bacteria were probably introduced into the reservoir as it was being exploited. This work provides significant information on the structure of bacterial communities in high‐temperature, long‐term water‐flooded petroleum reservoirs.