Open AccessGenome mapping of Arcobacter butzleri
Author(s) -
Stoeva Kalina,
Bruce Ward F.
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00135.x
Subject(s) - biology , campylobacter jejuni , genome , arcobacter , gene , genetics , 23s ribosomal rna , xhoi , microbiology and biotechnology , campylobacter , 16s ribosomal rna , bacteria , bamhi , ribosome , rna
A genome map of the potential food‐poisoning pathogen Arcobacter butzleri , strain NCTC 12481, has been constructed. The map was ordered by a combination of one‐ and two‐dimensional pulsed field gel electrophoresis, using the restriction enzymes Eag I, Sac II, Sal I and Xho I, and Southern hybridization with digoxygenin‐labelled probes. The size of the genome was estimated at 2.57±0.01 Mb; this is 0.9 Mb larger than the genome of the closely related species Campylobacter jejuni . We deduce that there are five copies of the 23S and 16S rRNA genes present in the A . butzleri NCTC 12481 genome and two copies of the gene glyA . A gene homologous to pglF , involved in bacterial glycosylation in C. jejuni , was also located on the genome map. In C. jejuni and Wolinella succinogenes , most glycosylation genes are clustered together and the gene order is similar. In A. butzleri NCTC 12481, no analogous organization was found in the region sequenced.