Open AccessMolecular cloning, characterization, and expression studies of a novel chitinase gene ( ech30 ) from the mycoparasite Trichoderma atroviride strain P1
Author(s) -
Klemsdal Sonja S.,
Clarke Jihong Liu,
Hoell Ingunn A.,
Eijsink Vincent G. H.,
Brurberg MayBente
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00132.x
Subject(s) - chitinase , botrytis cinerea , biology , gene , cloning (programming) , molecular cloning , sequence analysis , microbiology and biotechnology , chitin , trichoderma , nucleic acid sequence , gene expression , genetics , biochemistry , botany , chitosan , computer science , programming language
We describe the cloning and characterization of a single copy gene from Trichoderma atroviride P1 encoding a novel 30 kDa chitinase, Ech30. Ech30 is a family 18 chitinase showing low sequence similarity to other Trichoderma chitinases. Real‐time quantitative RT‐PCR studies revealed that expression of the ech30 gene was induced by the presence of Botrytis cinerea in plate confrontation assays, but hardly by chitin in liquid cultures. Studies of Ech30 purified from an Escherichia coli strain overexpressing the ech30 gene devoid of the leader sequence and a predicted intron, showed that the gene encodes an active chitinase, which, as expected for family 18 chitinases, is inhibited by allosamidin.