Open AccessAn EST library from Puccinia graminis f. sp. tritici reveals genes potentially involved in fungal differentiation
Author(s) -
Broeker Katja,
Bernard Frank,
Moerschbacher Bruno M.
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00127.x
Subject(s) - biology , puccinia , suppression subtractive hybridization , rust (programming language) , stem rust , expressed sequence tag , fungus , pathogen , botany , gene , cdna library , mycelium , microbiology and biotechnology , host (biology) , complementary dna , genetics , mildew , computer science , programming language
The rust fungus Puccinia graminis f. sp. tritici is an obligately biotrophic pathogen on wheat plants and thus difficult to investigate. Hence, little is known about this fungus at the molecular level. We constructed a differential suppression subtractive hybridization cDNA‐library from rust‐infected vs. healthy wheat plants. The majority of expressed sequence tags (ESTs) showed similarities to fungal sequences. Semiquantitative RT‐PCR using mRNA from rust‐infected leaves, and from axenically grown, differentiating and nondifferentiating young rust colonies as well as sporulating and nonsporulating mature mycelia revealed rather diverse expression patterns for different ESTs, shedding new light on their potential involvement in differentiation and host–pathogen interaction.