Open AccessThe putative ABC transporter YheH/YheI is involved in the signalling pathway that activates KinA during sporulation initiation
Author(s) -
Fukushima Sanae,
Yoshimura Mika,
Chibazakura Taku,
Sato Tsutomu,
Yoshikawa Hirofumi
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00104.x
Subject(s) - kinase , mutant , biology , atp binding cassette transporter , yeast , microbiology and biotechnology , gene , promoter , phosphorylation , signal transduction , genetics , biochemistry , transporter , gene expression
The primary kinases that control the supply of phosphate to the phosphorelay are KinA and KinB, although it is not yet known what type of signal(s) activates these kinases. Our systematic study of protein‐protein interactions using yeast two‐hybrid analysis revealed an interaction between KinA and YheH. YheH with the preceding gene product YheI is categorized as an ABC transporter. Overexpression of yheH/yheI in the kinB mutant resulted in a reduced sporulation efficiency. Moreover, reporter assays using Spo0A∼P dependent promoters revealed that the deficiency in sporulation is probably due to a failure in the activation of Spo0A. Our results further suggest that the N‐terminal region of YheH may play an important role in sensing the signal to be delivered to the C‐terminally bound KinA.