Open AccessMunumbicins E‐4 and E‐5: novel broad‐spectrum antibiotics from Streptomyces NRRL 3052
Author(s) -
Castillo Uvidelio F.,
Strobel Gary A.,
Mullenberg Kirby,
Condron Margaret M.,
Teplow David B.,
Folgiano Vincenzo,
Gallo Monica,
Ferracane Rosalia,
Mannina Luisa,
Viel Stepanie,
Codde Marissa,
Robison Richard,
Porter Heide,
Jensen James
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2005.00080.x
Subject(s) - streptomyces , endophyte , antibiotics , biology , microbiology and biotechnology , bacteria , antibacterial activity , plasmodium falciparum , botany , genetics , malaria , immunology
Streptomyces NRRL 30562 was originally isolated as an endophyte from Kennedia nigriscans , snakevine, in the Northern Territory of Australia. This plant has been used for centuries by Aboriginal peoples to treat open bleeding wounds to prevent sepsis. A solvent extract of the crude fluid from cultures of this endophyte possesses wide‐spectrum antibiotic activity. Some of the bioactivity is associated with the appearance of actinomycins X2, D, and Xoβ, the first two of which had been previously designated munumbicins A and B, respectively. Other novel compounds bearing wide‐spectrum antibiotic activity are also produced by Streptomyces NRRL 30562, and these are designated munumbicins E‐4 and E‐5. Mass spectrometric analyses of these peptide antibiotics show that they have identical masses (1445.00) but different retention times on HPLC. Both compounds showed activity against gram‐positive and gram‐negative bacteria. The plant pathogenic fungus, Pythium ultimum is sensitive to both munumbicins at 5.0 μg mL −1 The malarial parasite, Plasmodium falciparum has IC 50 values of 0.50±0.08 and 0.87±0.0.26 μg mL −1 for E‐4 and E‐5, respectively. It appears that other bioactive compounds, related to E‐4 and E‐5, are also produced making it the most biologically active endophytic Streptomyces spp. on record.