Open AccessComputational identification of BioR, a transcriptional regulator of biotin metabolism in Alphaproteobacteria , and of its binding signal
Author(s) -
Rodionov Dmitry A.,
Gelfand Mikhail S.
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2005.00070.x
Subject(s) - biology , alphaproteobacteria , gammaproteobacteria , biochemistry , gene , 16s ribosomal rna
Comparative genomic analysis was applied to identify the biotin transcriptional regulator, BioR, in most Alphaproteobacteria , and to identify its recognition signal TTATMKATAA. BioR belongs to the GntR family of transcriptional repressors. The functional assignment is supported by three lines of evidence: (1) bioR is positionally clustered with various bio genes, both for biotin biosynthesis and transport; (2) in most cases, candidate BioR‐binding sites (BIOR boxes) are observed upstream of the bioR genes, suggesting autoregulation; (3) the phyletic distribution of the BIOR boxes coincides exactly with the phyletic distribution of the bioR genes, as the genomes lacking BIOR boxes do not have orthologs of bioR . Thus, in Alphaproteobacteria , BioR seems to have assumed the role of the biotin regulator that in most other bacteria is fulfilled by the dual function biotin–protein ligase BirA having the DNA‐binding helix‐turn‐helix domain.