Open AccessDevelopment of a rapid and simple Agrobacterium tumefaciens‐ mediated transformation system for the fungal pathogen Heterobasidion annosum
Author(s) -
Samils Nicklas,
Elfstrand Malin,
Lindner Czederpiltz Daniel L.,
Fahleson Jan,
Olson Åke,
Dixelius Christina,
Stenlid Jan
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2005.00069.x
Subject(s) - agrobacterium tumefaciens , heterobasidion annosum , transformation (genetics) , biology , pathogen , agrobacterium , microbiology and biotechnology , botany , genetics , gene , picea abies
Heterobasidion annosum causes root and butt‐rot in trees and is the most serious forest pathogen in the northern hemisphere. We developed a rapid and simple Agrobacterium ‐mediated method of gene delivery into H. annosum to be used in functional studies of candidate genes and for visualization of mycelial interactions. Heterobasidion annosum TC 32‐1 was cocultivated at pH 5.6 and 20°C in Hagems medium with Agrobacterium tumefaciens C58 carrying plasmids with hygromycin B resistance as the selectable marker and green fluorescent protein as a visual marker. We obtained 18 mitotically stable transformed isolates showing green fluorescence protein activity.