Open AccessIsolation and characterization of Halomonas sp. strain IMPC, a p ‐coumaric acid‐metabolizing bacterium that decarboxylates other cinnamic acids under hypersaline conditions
Author(s) -
Abdelkafi Slim,
Labat Marc,
Casalot Laurence,
Chamkha Mohamed,
Sayadi Sami
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2005.00061.x
Subject(s) - halophile , halomonas , protocatechuic acid , biology , cinnamic acid , biochemistry , p coumaric acid , microbiology and biotechnology , phenanthrene , bacteria , chemistry , organic chemistry , genetics , ferulic acid , antioxidant
A moderately halophilic, mesophilic, Gram‐negative, motile, nonsporulating bacterium, designated strain IMPC, was isolated from a table‐olive fermentation rich in aromatic compounds, after enrichment on p ‐coumaric acid under halophilic conditions. Strain IMPC was able to degrade p ‐coumaric acid. p ‐hydroxybenzaldehyde and p ‐hydroxybenzoic acid were detected as breakdown products from p ‐coumaric acid. Protocatechuic acid was identified as the final aromatic product of p ‐coumaric acid catabolism before ring fission. Strain IMPC transformed various cinnamic acids with substituent H, OH, CH 3 or OCH 3 in the para ‐ and/or meta ‐position of the aromatic ring to the corresponding benzoic acids, indicating a specific selection. A β‐oxidation pathway was proposed for these transformations. Phylogenetic analysis of the 16S rRNA gene revealed that this isolate was a member of the genus Halomonas . Strain IMPC was closely related to Halomonas elongata ATCC 33173 T and Halomonas eurihalina ATCC 49336 T .