Open AccessStrain typing with IS Lpl1 in lactobacilli
Author(s) -
Petrovic Tanja,
Niksic Miomir,
Bringel Françoise
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2005.00042.x
Subject(s) - lactobacillus plantarum , typing , biology , lactobacillus casei , strain (injury) , microbiology and biotechnology , lactobacillus , 16s ribosomal rna , gene , bacteria , genetics , lactic acid , anatomy
Twenty‐seven Lactobacillus plantarum ssp. plantarum , 11 Lactobacillus paraplantarum and five Lactobacillus casei ‐related strains, isolated from various autochthonous Serbian and Montenegro‐fermented foods, were identified using phenotypical characterization and current PCR methods based on PCR of the recA gene or the 23S–5S rRNA gene intragenic spacer (IS) region. The strains were genotypically characterized by a new method based on the insertion sequence element IS Lpl11 that grouped these lactobacilli into 10 IS‐fingerprinting groups. Between six and 23 copies of the IS Lpl1 were found in each strain and the IS Lpl1 ‐fingerprint groups correlated well with the origin of the strains. The method proved suitable for strain typing of lactic acid bacteria at the infraspecies level.