Open AccessInteraction analysis of the AAA ATPase TAA43 by the bacterial two‐hybrid system
Author(s) -
Santos Leticia,
Zwickl Peter
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2005.00032.x
Subject(s) - thermoplasma acidophilum , biology , atpase , aaa proteins , biochemistry , ribosomal protein , ribosomal rna , rna , microbiology and biotechnology , ribosome , gene , enzyme
The TAA43 ATPase of Thermoplasma acidophilum , an archaeal member of the AAA protein family, is known to have an atypical oligomeric state and a nonspecific association with high‐molecular‐weight protein complexes. We assessed the in vivo binding pattern of TAA43 using the bacterial two‐hybrid system. We found 36 positive isolates interacting with TAA43. Our analysis showed that TAA43 interacts preferentially with nonribosomal proteins containing ribosomal domains and regions involved in RNA metabolism.