Open AccesssucAB and sucCD are mutually essential genes in Escherichia coli
Author(s) -
Yu Byung Jo,
Sung Bong Hyun,
Lee Ju Young,
Son Sung Hwa,
Kim Mi Sun,
Kim Sun Chang
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2005.00026.x
Subject(s) - escherichia coli , biology , arabinose , gene , enzyme , biochemistry , fermentation , xylose
sucAB and sucCD of Escherichia coli encode enzymes that generate succinyl‐CoA from 2‐oxoglutarate and succinate, respectively. Their mutual essentiality was studied. sucAB and sucCD could be deleted individually, but not simultaneously. The mutual essentiality of sucAB and sucCD was further confirmed by the conditional expression of sucABCD , sucAB , and sucCD under the control of a P BAD in E. coli MG1655, E. coli MG1655 (Δ sucCD ), and E. coli MG1655 (Δ sucAB ), respectively. These strains grew well in Luria–Bertani medium containing 0.1% arabinose, but not in the absence of arabinose unless the medium was supplemented with succinyl‐CoA. Our results indicate that either sucAB or sucCD is enough to produce succinyl‐CoA that is essential for cell viability.