Open AccessBiodiversity amongst cultivable polycyclic aromatic hydrocarbon‐transforming bacteria isolated from an abandoned industrial site
Author(s) -
Zocca Chiara,
Gregorio Simona,
Visentini Filippo,
Vallini Giovanni
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2004.tb09779.x
Subject(s) - temperature gradient gel electrophoresis , bacteria , microbial population biology , polycyclic aromatic hydrocarbon , gel electrophoresis , biology , microbiology and biotechnology , environmental chemistry , chemistry , biochemistry , 16s ribosomal rna , genetics
Abstract The characterisation of a microbial community of a polycyclic aromatic hydrocarbons (PAHs) contaminated site (formerly Carbochimica, Trento, Italy) was carried out. A preliminary evaluation of the heterogeneity and the metabolic activity of the microbial community were attempted by denaturing gradient gel electrophoresis (DGGE) and reverse transcriptase‐denaturing gel electrophoresis (RT‐DGGE). The presence of a heterogeneous and metabolically active microbial community was found. To evaluate the PAH‐transforming potential of the soil bacterial community, enrichment cultures were set up. Taxonomically diverse bacteria, showing different biochemical PAH‐transforming pathways were obtained. Some of the isolates showed not nah ‐homologous PAH‐transforming genotypes.