Open AccessNovel strains of Salmonella typhimurium as potential vectors for gene delivery
Author(s) -
Michael Agnieszka,
Stratford Richard,
Khan Shahid,
Dalgleish Angus,
Pandha Hardev
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2004.tb09775.x
Subject(s) - salmonella , microbiology and biotechnology , enterobacteriaceae , biology , gene delivery , gene , bacteria , genetics , escherichia coli , genetic enhancement
DNA vaccines are known to induce long‐term antigen specific cellular responses. We tested two new strains of Salmonella typhimurium , one carrying a mutation in a SPI‐2 gene and the aroC ‐gene and another carrying mutations in the s ifA ‐ and aroC ‐genes, as potential DNA vaccine delivery vehicles. We compared them with the SL7207 strain and found that the new strains were more invasive, and that they were efficient mediators of gene transfer in vitro using EGFP as reporter gene. We tested the ability of the new strains to survive within the spleen, liver and mesenteric lymph nodes and evaluated their safety in C57/BL/6J mice.