Open AccessInfluence of ethyl acetate production and ploidy on the anti‐mould activity of Pichia anomala
Author(s) -
Fredlund Elisabeth,
Druvefors Ulrika Ädel,
Olstorpe Matilda Nilsson,
Passoth Volkmar,
Schnürer Johan
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2004.tb09747.x
Subject(s) - penicillium roqueforti , ethyl acetate , food science , anomala , strain (injury) , biology , yeast , food spoilage , bioassay , chemistry , water activity , botany , biochemistry , penicillium , ecology , bacteria , genetics , geotechnical engineering , anatomy , engineering , water content
A diploid and a haploid strain of Pichia anomala were tested for their biocontrol ability against the spoilage mould Penicillium roqueforti in glass tubes filled with grain at two water activities ( a w ). At a w 0.98, the two yeast strains grew and inhibited mould growth equally well and showed similar patterns of ethyl acetate production, reaching maximum values of 10–14 μg ml −1 headspace. At a w 0.95, both growth and biocontrol performance of the haploid strain were reduced. Ethyl acetate formation was also substantially reduced, with maximum headspace concentrations of 4 μg ml −1 . We conclude that ethyl acetate is a major component of the anti‐mould activity. The inhibitory effect of ethyl acetate was confirmed in a bioassay where the pure compound reduced biomass production of P. roqueforti .