Open AccessThe interaction of Pseudomonas aeruginosa PAK with human and animal respiratory tract cell lines
Author(s) -
Hambrook Joy,
Titball Richard,
Lindsay Christopher
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2004.tb09736.x
Subject(s) - pseudomonas aeruginosa , pilus , respiratory tract , microbiology and biotechnology , cell culture , biology , virulence , in vitro , cell , a549 cell , virulence factor , pathogen , cell type , pseudomonadaceae , bacteria , respiratory system , gene , genetics , anatomy
A major virulence factor of a common human pathogen Pseudomonas aeruginosa , was investigated to determine if it dominated attachment interactions in a variety of in vitro cell culture systems. It was found that Type‐IV pilus‐type mechanisms, which mediated the attachment of P. aeruginosa to three human respiratory tract cell lines (A549, BEAS‐2B and RPMI 2650) also mediated attachment to two respiratory tract cell lines from mouse (C57) and rat (L‐2) to a similar degree. Significant differences were found in the number of P. aeruginosa associated with the human, rat and mouse cell lines. Additionally, differences were also found between A547, C57 and L‐2 cells with respect to the moieties that P. aeruginosa interacted with at the level of the cell surface, suggesting that asialo‐GM1 ligands were not the only structure that this bacterium could interact with in order to associate with host cells.