Open AccessA novel glutamate‐dependent acid resistance among strains belonging to the Proteeae tribe of Enterobacteriaceae
Author(s) -
Park Geun woo,
DiezGonzalez Francisco
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2004.tb09711.x
Subject(s) - providencia , microbiology and biotechnology , proteus , glutamate decarboxylase , enterobacteriaceae , bacteria , biology , escherichia coli , glutamic acid , glutamate receptor , biochemistry , amino acid , chemistry , gene , enzyme , genetics , receptor
Morganella, Providencia and Proteus strains were capable of surviving pH 2.0 for 1 h if glutamate was present. These strains did not have glutamic acid decarboxylase activity and the gadAB genes were not detected in any of these bacteria. When exposed to pH 2.0 acid shocks, the survival rate of these bacteria was significantly increased with glutamate concentrations as low as 0.3 mM in the acid media. Escherichia coli cells incubated at pH 3.4 consumed four times more glutamate and produced at least 7‐fold more γ‐amino butyric acid than Morganella, Providencia and Proteus strains. These results indicate that strains belonging to the Proteeae tribe might have novel glutamate dependent acid‐resistance mechanisms.