Open AccessGrowth phase‐dependent expression of an endoglucanase encoding gene ( eglS ) in Streptomyces rochei A2
Author(s) -
Tamburini Elena,
Perito Brunella,
Mastromei Giorgio
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2004.tb09706.x
Subject(s) - biology , gene expression , streptomyces , gene , streptomycetaceae , rna , actinomycetales , strain (injury) , cellulase , cell growth , microbiology and biotechnology , genetics , biochemistry , bacteria , enzyme , anatomy
Different growth phases of Streptomyces rochei A2, a cellulolytic strain isolated from termite gut, were determined in liquid culture. The strain exhibited a multiphasic growth curve typical of streptomycetes. In S. rochei A2, the secreted endoglucanase EglS is coded by a TTA‐containing gene, conserved in other Streptomyces . The onset of S. rochei A2 eglS gene expression was determined by RT‐PCR using total RNA extracted at different growth phases. The eglS gene was expressed only during transition, second rapid growth and stationary phases, the timing of expression being transcriptionally regulated.