Open AccessMicroplate screening assay for the detection of arsenite‐oxidizing and arsenate‐reducing bacteria
Author(s) -
Simeonova Diliana D.,
Lièvremont Didier,
Lagarde Florence,
Muller Daniel A.E.,
Groudeva Veneta I.,
Lett MarieClaire
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2004.tb09703.x
Subject(s) - arsenite , arsenic , arsenate , chemistry , oxidizing agent , bacteria , bioremediation , chromatography , silver nitrate , colorimetry , colorimetric analysis , environmental chemistry , nuclear chemistry , biology , organic chemistry , genetics
An efficient, inexpensive microplate colorimetric assay for screening of bacteria which can be used in bioremediation of arsenic was developed. The assay is based on the colorimetric analysis of the precipitates formed upon reaction of silver nitrate with arsenic. The method proved reliable and sensitive for the detection of As[III] oxidizers and As[V] reducers and can be used over a large pH range (5.8–8.4). Seventy‐eight bacterial strains isolated from different environments were tested by this method. It also showed agreement with results obtained by high‐performance liquid chromatography coupled with inductively coupled plasma atomic emission spectrometry.