Open AccessSimple and rapid PCR method for identification of streptococcal species relevant to animal infections based on 23S rDNA sequence
Author(s) -
Kawata Koji,
Anzai Toru,
Senna Kazuhiro,
Kikuchi Naoya,
Ezawa Ayako,
Takahashi Tatsuhumi
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2004.tb09678.x
Subject(s) - streptococcus dysgalactiae , biology , microbiology and biotechnology , polymerase chain reaction , identification (biology) , canis , virology , 23s ribosomal rna , streptococcus agalactiae , streptococcus , genetics , bacteria , gene , rna , ribosome , paleontology , botany
A PCR identification system targeting 23S rDNA sequences for the identification of eight streptococcal species relevant to animal infections ( Streptococcus agalactiae, S. bovis, S. canis, S. dysgalactiae, S. equi, S. porcinus, S. suis and S. uberis ) was developed. This system consists of two PCR reactions, A and B, in which seven and eight primers, respectively, are used simultaneously, and was designed so that each amplification product indicates a species by its size. A total of 111 cultures, including the type strain of eight species, could be successfully identified and differentiated as individual species, except for the cross reactivity between S. bovis and S. equinus . The developed PCR system can complete the identification procedure for eight streptococcal species through two tube reactions per isolate, and, therefore, might provide a rapid, simple and accurate diagnostic tool for veterinary laboratories.