Open AccessThree fur homologues from Anabaena sp. PCC7120: exploring reciprocal protein‐promoter recognition
Author(s) -
Hernández José A,
LópezGomollón Sara,
Bes M.Teresa,
Fillat María F,
Peleato M.Luisa
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2004.tb09658.x
Subject(s) - promoter , biology , gene , recombinant dna , open reading frame , escherichia coli , dna binding protein , anabaena , dna , genetics , microbiology and biotechnology , biochemistry , peptide sequence , transcription factor , cyanobacteria , gene expression , bacteria
DNA sequence analysis of the Anabaena sp. PCC7120 genome confirmed the presence of three open reading frames ( all1691, all2473 and alr0957 ) containing the histidine‐rich region characteristic of the Fur family. The genes coding for the three Fur proteins were cloned and overexpressed in Escherichia coli . The overexpression products, called FurA, FurB and FurC are only distantly related. The ability of the three recombinant proteins to bind iron‐boxes identified in the three fur promoter regions was tested by electrophoretical mobility shift assays. FurA binds the three fur promoters with increased affinity in presence of metal. FurB also binds the three fur promoters, and its affinity is increased with DTT. FurC does not bind to furA or furB promoter regions or to its own promoter. However, FurC affects the ability of FurB and FurA to bind their target promoters.